The Journal of Phytopharmacology 2023; 12(6):377-391 ; DOI:10.31254/phyto.2023.12604
Varietal level comparison of Moringa oleifera Lam. for phytochemical constituents and antifungal properties against Fusarium oxysporum and Albugo candida
1. Research Scholar Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India
2. Associate Professor, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India
*Author to whom correspondence should be addressed.
Received: 3rd September, 2023 / Accepted: 20th November, 2023 / Published : 30th December, 2023
Background: The main objective of this work was to examine the chemical components, antioxidant and antifungal activities of an ethanolic extract of three Moringa oleifera varieties (PKM-1, PKM-2, and ODC-3) against selected fungal strains. The ethanolic extract was prepared using the Soxhlet extraction method. The extracts of three different varieties were tested for antioxidant activity as well as antifungal activity against Fusarium oxysporum and Albugo candida using the disc diffusion method, and minimum inhibitory concentration (MIC) values were determined using the micro dilution method. Using the FTIR technique, spectrophotometer equipment was used to identify the distinctive peak values and functional groups. HPLC and GCMS were used to determine the phytochemical profiles of the three variants. Results: The preliminary phytochemical investigation revealed the presence of numerous secondary metabolites in all three M. oleifera types. The PKM-1 cultivar had a considerably higher total phenolic content (99.93 ± 0.239 mg GAE /g) than the PKM-2 and ODC-3 varieties of M. oleifera (81.58 ± 0.369 and 91.73 ± 0.254 mg GAE/g). The flavonoid content of the PKM-1 variety (135.3 ± 0.494 mg QE/g) was maximum than that of the PKM-2 and ODC-3 varieties (106.9 ± 0.553 and 117.1 ± 0.413 mg QE/g). In the DPPH and H2O2 assays, the PKM-1 variant was the most potent inhibitor (IC50) compared to the other two varieties. Antifungal activity testing demonstrated that all the three varieties suppress the growth of both the fungal strains such as F. oxysporum and A. candida with a mean zone of inhibition. FTIR examination of ethanol extract of Moringa types (PKM-1, PKM-2, and ODC-3) revealed the presence of distinct functional chemical classes at a variety of characteristic bands. GCMS analysis confirmed the presence of 25 metabolites in M. oleifera PKM-1 and 23 compounds in PKM-2 varieties. Conclusion: The results showed that M. oleifera has a diverse set of phytoconstituents with considerable antioxidant and antifungal effects, confirming its applicability in a wide range of applications.
Albugo candida, Antioxidants, Antifungal, Bioactive compounds, Fusarium oxysporum, Moringa oleifera, Phytoconstituents
HOW TO CITE THIS ARTICLE
Yadav S, Mathur J. Varietal level comparison of Moringa oleifera Lam. for phytochemical constituents and antifungal properties against Fusarium oxysporum and Albugo candida. J Phytopharmacol 2023; 12(6):377-391. doi: 10.31254/phyto.2023.12604
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